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KMID : 0545119910010020126
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Journal of Microbiology and Biotechnology
1991 Volume.1 No. 2 p.126 ~ p.129
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High Density Cell Culture of Mucor sp. KCTC 8405P for Production of γ-Linolenic Acid in Fed-Batch Culture
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Park Jong-Hyun
Shin Hyun-Kyung
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Abstract
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In order to produce y-linolenic acid by Mucor sp. KCTC 8405P, the fungus was cultivated in fed-batch culture with two phases, i.e., growth in yeast-like form and induction to hyphal growth by pH shift of the culture medium during cultivation. The synchronous growth of the fungus into the appropriate sizes was important for the high density cell culture of this dimorphic fungus. Dissolved oxygen concentration in the medium did not affect degree of unsaturation of fatty acids and γ-linolenic acid content. Under the culture conditions applied in this experiment, the fungus was found to produce 100g/ℓ dry mycelia containing 40% of the lipids, where γ-linolenic acid comprised about 9% of the total extractable fatty acids.
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